Počet záznamů: 1  

M-CSFR/CSF1R signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands

  1. 1.
    0558018 - ÚMG 2023 RIV US eng J - Článek v odborném periodiku
    Hason, Martina - Mikulášová, Tereza - Machoňová, Olga - Pombinho, António R. - van Ham, T.J. - Irion, U. - Nuesslein-Volhard, C. - Bartůněk, Petr - Svoboda, Ondřej
    M-CSFR/CSF1R signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands.
    Blood Advances. Roč. 6, č. 5 (2022), s. 1474-1488. ISSN 2473-9529. E-ISSN 2473-9537
    Grant CEP: GA MŠMT(CZ) LM2018131; GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF18_046/0016045; GA ČR(CZ) GA18-18363S; GA MŠMT(CZ) LM2018130
    Institucionální podpora: RVO:68378050
    Klíčová slova: colony-stimulating factor-1 * in-vitro * macrophage * fms * cells * il-34 * csf1r * granulocytes * progenitor * expression
    Obor OECD: Hematology
    Impakt faktor: 7.6, rok: 2022
    Způsob publikování: Open access
    https://ashpublications.org/bloodadvances/article/6/5/1474/483305/M-CSFR-CSF1R-signaling-regulates-myeloid-fates-in

    Macrophage colony-stimulating factor receptor (M-CSFR/CSF1R) signaling is crucial for the differentiation, proliferation, and survival of myeloid cells. The CSF1R pathway is a promising therapeutic target in many human diseases, including neurological disorders and cancer. Zebrafish are commonly used for human disease modeling and preclinical therapeutic screening. Therefore, it is necessary to understand the proper function of cytokine signaling in zebrafish to reliably model human-related diseases. Here, we investigate the roles of zebrafish Csf1rs and their ligands (Csf1a, Csf1b, and Il34) in embryonic and adult myelopoiesis. The proliferative effect of exogenous Csf1a on embryonic macrophages is connected to both receptors, Csf1ra and Csf1rb, however there is no evident effect of Csf1b in zebrafish embryonic myelopoiesis. Furthermore, we uncover an unknown role of Csf1rb in zebrafish granulopoiesis. Deregulation of Csf1rb signaling leads to failure in myeloid differentiation, resulting in neutropenia throughout the whole lifespan. Surprisingly, Il34 signaling through Csf1rb seems to be of high importance as both csf1rb(Delta 4bP)-deficient and il34(Delta 5bp)-deficientzebrafish larvae lack granulocytes. Our single-cell RNA sequencing analysis of adult whole kidney marrow (WKM) hematopoietic cells suggests that csf1rb is expressed mainly by blood and myeloid progenitors, and the expression of csf1ra and csf1rb is nonoverlapping. We point out differentially expressed genes important in hematopoietic cell differentiation and immune response in selected WKM populations. Our findings could improve the understanding of myeloid cell function and lead to the further study of CSF1R pathway deregulation in disease, mostly in cancerogenesis.
    Trvalý link: http://hdl.handle.net/11104/0331932

     
     
Počet záznamů: 1  

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