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The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations
- 1.0557670 - ÚFCH JH 2023 RIV CH eng J - Článek v odborném periodiku
Schicht, M. - Riedlová, Kamila - Kukulka, M. - Li, W. - Scheer, A. - Garreis, F. - Jacobi, Ch. - Paulsen, F. - Cwiklik, Lukasz - Bräuer, L.
The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations.
International Journal of Molecular Sciences. Roč. 23, č. 10 (2022), č. článku 5783. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA21-19854S
Institucionální podpora: RVO:61388955
Klíčová slova: tear film * surfactant protein * surface tension
Obor OECD: Physical chemistry
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film. View Full-Text
Trvalý link: http://hdl.handle.net/11104/0331578
Název souboru Staženo Velikost Komentář Verze Přístup 0557670.pdf 3 2.7 MB open access Vydavatelský postprint povolen
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