Liquid biopsy in cancer research – Development of a new 3D printed device for sample pretreatment

0557529 - ÚIACH 2023 CZ eng A - Abstrakt
Hrušková, Helena - Řemínek, Roman - Foret, František
Liquid biopsy in cancer research – Development of a new 3D printed device for sample pretreatment.
Czech Chemical Society Symposium Series. Roč. 20, “Amerika” 21st (2022), s. 16-16. ISSN 2336-7202.
[Interdisciplinary Meeting of Young Researchers and Students in the field of chemistry, biochemistry and molecular biology and biomaterials /21./. 16.05.2022-19.05.2022, Milovy]
Grant CEP: GA TA ČR(CZ) FW02020209
Institucionální podpora: RVO:68081715
Klíčová slova: printed device for sample pretreatment * cancer research * DNA separation
Obor OECD: Analytical chemistry
http://www.interdisciplinarymeeting.cz/index.php

In the presented study, we developed a preparative device for the separation of short DNA fragments with less than 1000 base pairs. This precleaning should result in higher specificity and sensitivity of the subsequent DNA analyses. The design of the device enables the parallel separation of multiple samples. For the fabrication, 3D printing was used, providing fast and low-cost manufacturing of the product. The device is based on electrodriven separation methods. These techniques enable the partition of DNA fragments in an external electric field. The separation itself is mediated by the sieving effect of the separation media and/or difference in the analyte’s migration velocities. At the end of the separation process, the precleaned fraction can be collected from the device, and DNA analyses performed.
Trvalý link: http://hdl.handle.net/11104/0331495