Specialist Aphid Feeding Causes Local Activation of Salicylic and Jasmonic Acid Signaling in Arabidopsis Veins

0556930 - ÚEB 2023 RIV US eng J - Článek v odborném periodiku
Rubil, Nikoleta - Kalachova, Tetiana - Hauser, T. P. - Burketová, Lenka
Specialist Aphid Feeding Causes Local Activation of Salicylic and Jasmonic Acid Signaling in Arabidopsis Veins.
Molecular Plant-Microbe Interactions. Roč. 35, č. 2 (2022), s. 119-124. ISSN 0894-0282. E-ISSN 1943-7706
Grant CEP: GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) LTC17013; GA MŠMT(CZ) EF16_019/0000738
Institucionální podpora: RVO:61389030
Klíčová slova: Arabidopsis thaliana * Brevicoryne brassicae * callose * infestation * jasmonic acid * phytohormonal signaling * salicylic acid
Obor OECD: Cell biology
Impakt faktor: 3.5, rok: 2022
Způsob publikování: Open access
http://doi.org/10.1094/MPMI-08-21-0203-SC

Aphids, the phloem sap feeders, probe into leaf tissues and activate a complex network of plant defense responses. Phytohormonal signaling plays a major role in this network, however, the dynamics of the signal spreading is yet to be clarified. Despite the growing knowledge about transcriptomic changes upon infestation, results often differ due to sampling, varying strongly between the tissues collected at the single feeding site, individual leaves, pooled infested leaves, or whole plant rosettes. This study focuses on activation of salicylic acid (SA) and jasmonic acid (JA) signals in Arabidopsis leaves during infestation by cabbage aphid (Brevicoryne brassicae) in high spatio-temporal resolution. We used genetically encoded fluorescent biosensors, histochemistry, and quantitative reverse transcription-PCR to precisely map activation of distinct branches of phytohormonal signaling. We found a rapid induction of SA and JA signaling markers in cells surrounding stylet puncture, colocalizing with callose deposition. For both PR1 and JAZ10, we detected activation at 24 h postinfestation (hpi), increasing and spreading along the veins until 72 hpi and, to a lesser extent, within the epidermal pavement cells. The SA signaling wave appeared in parallel with JA-associated signaling and continued to increase in time. Our results first show a local activation of SA- and JA-related responses after stylet penetration of Arabidopsis leaves and bring a detailed insight into the spatio-temporal complexity of plant defense activation during specialist aphid attack.
Trvalý link: http://hdl.handle.net/11104/0331048