Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds

0556913 - ÚIACH 2024 RIV DE eng J - Článek v odborném periodiku
Václavek, Tomáš - Foret, František
Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds.
Electrophoresis. Roč. 44, 1-2 (2023), s. 313-322. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA MŠMT EF16_026/0008446
Institucionální podpora: RVO:68081715
Klíčová slova: capillary-electrophoresis * electrospray * separation * bioanalysis * mass spectrometry * microfluidics * single-cell analysis
Obor OECD: Analytical chemistry
Impakt faktor: 2.9, rok: 2022
Způsob publikování: Omezený přístup

Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates.
Trvalý link: http://hdl.handle.net/11104/0331026