Nanoaggregate-forming lipid-conjugated AS1411 aptamer as a promising tumor-targeted delivery system of anticancer agents in vitro'

0555750 - BFÚ 2022 RIV US eng J - Článek v odborném periodiku
Carvalho, J. - Lopes-Nunes, J. - Vialet, B. - Rosado, T. - Gallardo, E. - Vale, J. - Eloy, C. - Ferreira, S. - Palmeira-de-Oliveira, R. - Campello, M. P. C. - Paulo, A. - Barthelemy, P. - Mergny, Jean-Louis - Salgado, G.F. - Queiroz, J.A. - Ellington, A. - Cruz, C.
Nanoaggregate-forming lipid-conjugated AS1411 aptamer as a promising tumor-targeted delivery system of anticancer agents in vitro'.
Nanomedicine: Nanotechnology, Biology and Medicine. Roč. 36, AUG 2021 (2021), č. článku 102429. ISSN 1549-9634. E-ISSN 1549-9642
Grant CEP: GA MŠMT EF15_003/0000477
Institucionální podpora: RVO:68081707
Klíčová slova: g-quadruplex * surfactants * micelle * sirna * Nanoaggregates * Drug delivery * AS1411 G-quadruplex * Cervical cancer * hpv
Obor OECD: Other medical science
Impakt faktor: 6.458, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S1549963421000721?via%3Dihub

Nanoparticles offer targeted delivery of drugs with minimal toxicity to surrounding healthy tissue and have great potential in the management of human papillomavirus (HPV)-related diseases. We synthesized lipid-modified AS1411 aptamers capable of forming nanoaggregates in solution containing Mg2+. The nanoaggregates presented suitable properties for pharmaceutical applications such as small size (100 nm), negative charge, and drug release. The nanoaggregates were loaded with acridine orange derivative C-8 for its specific delivery into cervical cancer cell lines and HPV-positive tissue biopsies. This improved inhibition of HeLa proliferation and cell uptake without significantly affecting healthy cells. Finally, the nanoaggregates were incorporated in a gel formulation with promising tissue retention properties aiming at developing a local delivery strategy of the nanoaggregates in the female genital tract. Collectively, these findings suggest that the nanolimnulation protocol has great potential for the delivery of both anticancer and antiviral agents, becoming a novel modality for cervical cancer management. (C) 2021 Elsevier Inc. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0330209