Fungal Endophytes of Vitis vinifera-Plant Growth Promoters or Potentially Toxinogenic Agents?

0555533 - MBÚ 2023 RIV CH eng J - Článek v odborném periodiku
Stránská, M. - Džuman, Z. - Prusova, M. - Behner, A. - Kolouchová, I. - Lovecká, P. - Řezanka, Tomáš - Kolařík, Miroslav - Hajšlová, J.
Fungal Endophytes of Vitis vinifera-Plant Growth Promoters or Potentially Toxinogenic Agents?
Toxins. Roč. 14, č. 2 (2022), č. článku 66. ISSN 2072-6651. E-ISSN 2072-6651
Výzkumná infrastruktura: METROFOOD-CZ - 90100
Institucionální podpora: RVO:61388971
Klíčová slova: fusarium-proliferatum * biocontrol agent * mycotoxins * alkaloids * microscopic filamentous fungi * endophytes * mycotoxins * liquid chromatography * mass spectrometry
Obor OECD: Microbiology
Impakt faktor: 4.2, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2072-6651/14/2/66

Fungal endophytes occurring in grapevine (Vitis vinifera L.) are usually important sources of various compounds with biological activities with great potential for use in agriculture. Nevertheless, many species isolated from this plant belong to the genera Fusarium, Alternaria, or Aspergillus, all of which are well-known to produce mycotoxins. Our study is focused on the assessment of the toxinogenic potential of fungal endophytes isolated from vineyards in the Czech Republic. In total, 20 endophytic fungal species were cultivated in wine must, and 57 mycotoxins of different classes were analysed by liquid chromatography coupled with mass spectrometry. As a result, alternariol, tentoxin, meleagrin, roquefortine C, gliotoxin, and verruculogen were detected in the culture medium, of which verruculogen followed by gliotoxin were the most frequent (present in 90 and 40% of samples, respectively) and most concentrated (up to thousands ng/mL). The alternaria mycotoxins alternariol and tentoxin were detected not only in Alternaria sp. cultures, but traces of these mycotoxins were also quantified in the Diatripe and Epicoccum cultures. Meleagrin and roquefortine C were detected in Didymella sancta and Penicillium crustosum, gliotoxin was detected in Alternaria sp., Didymella sp., Aureobasidium pullulans, Cladosporium herbarum, Penicillium crustosum and Pleurophoma ossicola, and verruculogen was quantified in 99% of endophytic isolates investigated. The potential of endophytes to produce mycotoxins should be carefully checked, specifically in cases where they are intended for the purpose of V. vinifera growing.
Trvalý link: http://hdl.handle.net/11104/0330331