The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia

0555189 - BC 2022 RIV US eng J - Článek v odborném periodiku
Tortorelli, G. - Pettolino, F. - Lai, De Hua - Tomčala, Aleš - Bacic, A. - Oborník, Miroslav - Lukeš, Julius - McFadden, G.
The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia.
Journal of Phycology. Roč. 57, č. 6 (2021), s. 1805-1809. ISSN 0022-3646. E-ISSN 1529-8817
Grant CEP: GA MŠMT(CZ) EF16_019/0000759; GA ČR(CZ) GA21-03224S
Institucionální podpora: RVO:60077344
Klíčová slova: life-cycle * ultrastructure * dinoflagellate * morphology * evolution * Alveolata * calcofluor white * cell wall * cellulose * Chromera velia * chromerids * monosaccharide linkage analysis
Obor OECD: Microbiology
Impakt faktor: 3.173, rok: 2021
Způsob publikování: Open access
https://onlinelibrary.wiley.com/doi/10.1111/jpy.13211

Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features. These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans. There are two known species of chromerids: Chromera velia and Vitrella brassicaformis. Here, the biochemical composition of the C. velia cell wall was analyzed. Several polysaccharides adorn this structure, with glucose being the most abundant monosaccharide (approx. 80%) and predominantly 4-linked (approx. 60%), suggesting that the chromerids cell wall is mostly cellulosic. The presence of cellulose was cytochemically confirmed with calcofluor white staining of the algal cell. The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans. The present work provides, for the first time, insights into the outermost layers of the photosynthetic alveolate C. velia.
Trvalý link: http://hdl.handle.net/11104/0329712