Počet záznamů: 1
Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR
- 1.0555031 - BC 2022 RIV CH eng J - Článek v odborném periodiku
Stensvold, C.R. - Jirků-Pomajbíková, Kateřina - Tams, K. - Jokelainen, P. - Berg, R. P. K. D. - Marving, E. - Petersen, R. - Andersen, L.O. - Angen, Ø. - Nielsen, H.V.
Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR.
Microorganisms. Roč. 9, č. 6 (2021), č. článku 1189. E-ISSN 2076-2607
Institucionální podpora: RVO:60077344
Klíčová slova: genetic diversity * dientamoeba-fragilis * enterocytozoon-bieneusi * lawsonia-intracellularis * nursery pigs * blastocystis * prevalence * cryptosporidium * balantidium * population * metabarcoding * next-generation sequencing * zoonotic infections * parasite * parasitology * host specificity * genetic diversity * dna * pcr
Obor OECD: Microbiology
Impakt faktor: 4.926, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/2076-2607/9/6/1189
Several parasite species are shared between humans and pigs. We explored the application of next-generation sequencing-based metabarcoding supplemented with real-time PCR to fecal DNAs from 259 samples from 116 pigs in Denmark to detect and differentiate single-celled intestinal parasites of zoonotic relevance. Enterocytozoon bieneusi, Balantioides coli, and Giardia duodenalis were observed in 34/37 (92%), 148/259 (57%), and 86/259 (33%) samples, respectively. Entamoeba polecki ST1, E. polecki ST3, and Entamoeba hartmanni were detected in 104/259 (40%), 161/259 (62%), and 8/259 (3%) samples, respectively. Metabarcoding and real-time PCR detected Cryptosporidium in 90/259 (35%) and 239/259 (92%) of the samples, respectively, with Cryptosporidium suis and Cryptosporidium scrofarum observed in nearly equal proportions. Blastocystis subtypes 1, 3, 5, and 15 were found in 72 (28%), 6 (2%), 176 (68%), and 36 (14%) of 259 samples, respectively. Iodamoeba was identified in 1/259 samples (<1%), while none of 37 tested samples was positive for Dientamoeba fragilis. Our results illustrate how metabarcoding exemplifies a 'one-fits-many' approach to detecting intestinal single-celled parasites in feces supplemented with real-time PCR for selected parasites. Using metabarcoding with pathogen-specific assays may help detect emerging and previously underdetected pathogens and further elucidate the role of micro-eukaryotic parasites in human and animal health and disease.
Trvalý link: http://hdl.handle.net/11104/0329648
Počet záznamů: 1