Haem-responsive gene transporter enables mobilization of host haem in ticks

0554455 - BC 2022 RIV GB eng J - Článek v odborném periodiku
Perner, Jan - Hatalová, Tereza - Cabello-Donayre, M. - Urbanová, Veronika - Sojka, Daniel - Frantová, Helena - Hartmann, David - Jirsová, Dagmar - Perez-Victoria, J. M. - Kopáček, Petr
Haem-responsive gene transporter enables mobilization of host haem in ticks.
Open Biology. Roč. 11, č. 9 (2021), č. článku 210048. E-ISSN 2046-2441
Grant CEP: GA ČR(CZ) GA18-01832S; GA ČR(CZ) GA21-08826S; GA MŠMT(CZ) EF16_019/0000759; GA MŠMT(CZ) LM2018129
Institucionální podpora: RVO:60077344
Klíčová slova: structural basis * digest cells * iron * hemoglobin * metabolism * protein * midgut * recognition * degradation * sequence * ticks * hrg * transporter * haem * auxotrophy
Obor OECD: Parasitology
Impakt faktor: 7.000, rok: 2021
Způsob publikování: Open access
https://royalsocietypublishing.org/doi/10.1098/rsob.210048

Ticks, notorious blood-feeders and disease-vectors, have lost a part of their genetic complement encoding haem biosynthetic enzymes and are, therefore, dependent on the acquisition and distribution of host haem. Solute carrier protein SLC48A1, aka haem-responsive gene 1 protein (HRG1), has been implicated in haem transport, regulating the availability of intracellular haem. HRG1 transporter has been identified in both free-living and parasitic organisms ranging from unicellular kinetoplastids, nematodes, up to vertebrates. However, an HRG1 homologue in the arthropod lineage has not yet been identified. We have identified a single HRG1 homologue in the midgut transcriptome of the tick Ixodes ricinus, denoted as IrHRG, and have elucidated its role as a haem transporter. Data from haem biosynthesis-deficient yeast growth assays, systemic RNA interference and the evaluation of gallium protoporphyrin IX-mediated toxicity through tick membrane feeding clearly show that IrHRG is the bona fide tetrapyrrole transporter. We argue that during evolution, ticks profited from retaining a functional hrg1 gene in the genome because its protein product facilitates host haem escort from intracellularly digested haemoglobin, rendering haem bioavailable for a haem-dependent network of enzymes.
Trvalý link: http://hdl.handle.net/11104/0329166