Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness

0554391 - BC 2022 RIV US eng J - Článek v odborném periodiku
Nouzová, Marcela - Edwards, M. J. - Michalkova, V. - Ramirez, C.E. - Ruiz, M. - Areiza, M. - DeGennaro, M. - Fernandez-Lima, F. - Feyereisen, R. - Jindra, Marek - Noriega, F. G.
Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 118, č. 45 (2021), č. článku e2109381118. ISSN 0027-8424. E-ISSN 1091-6490
Grant CEP: GA ČR(CZ) GX20-05151X
Institucionální podpora: RVO:60077344
Klíčová slova: kruppel homolog 1 * methoprene-tolerant * corpus allatum * ovarian development * pathway * follicles * mosquitos * cyp15a1 * diptera * Aedes aegypti * corpora allata * juvenile hormone * methyl farnesoate * reproduction
Obor OECD: Zoology
Impakt faktor: 12.779, rok: 2021
Způsob publikování: Omezený přístup
https://www.pnas.org/content/118/45/e2109381118

Methyl farnesoate (MF) plays hormonal regulatory roles in crustaceans. An epoxidated form of MF, known as juvenile hormone (JH), controls metamorphosis and stimulates reproduction in insects. To address the evolutionary significance of MF epoxidation, we generated mosquitoes completely lacking either of the two enzymes that catalyze the last steps of MF/JH biosynthesis and epoxidation, respectively: the JH acid methyltransferase (JHAMT) and the P450 epoxidase CYP15 (EPDX). jhamt(-/-) larvae lacking both MF and JH died at the onset of metamorphosis. Strikingly, epox(-/-) mutants, which synthesized MF but no 1H, completed the entire life cycle. While epox(-/-) adults were fertile, the reproductive performance of both sexes was dramatically reduced. Our results suggest that although MF can substitute for the absence of JH in mosquitoes, it is with a significant fitness cost. We propose that MF can fulfill most roles of JH, but its epoxidation to JH was a key innovation providing insects with a reproductive advantage.
Trvalý link: http://hdl.handle.net/11104/0328993