Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition

0554307 - BC 2022 RIV AU eng J - Článek v odborném periodiku
Mazanec, Hynek - Koník, P. - Gardian, Zdenko - Kuchta, Roman
Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition.
International Journal for Parasitology. Roč. 51, č. 5 (2021), s. 327-332. ISSN 0020-7519. E-ISSN 1879-0135
Grant CEP: GA ČR(CZ) GX19-28399X; GA MŠMT(CZ) LM2015062
Institucionální podpora: RVO:60077344
Klíčová slova: Exosomes * Secretion * Proteins * Size exclusion chromatography * lc?ms * ms * Electron microscopy
Obor OECD: Marine biology, freshwater biology, limnology
Impakt faktor: 4.330, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0020751920303179?via%3Dihub

We provided the first known evidence of the presence and release of extracellular vesicles in adults of important model tapeworm Hymenolepis diminuta. Two different subtypes have been observed on the surface of the worm and among the secretory products confirmed by several microscopical methods. Proteomic analysis revealed the presence of parasite-specific proteins as well as those of the host in purified extracellular vesicles. Among the protein cargo, we identified potential drug targets, vaccine candidates and H. diminuta antigens. Finally, the protein composition further revealed proteins participating in the endosomal complex required for transport-dependent biogenesis pathway.
Trvalý link: http://hdl.handle.net/11104/0328941