Počet záznamů: 1
The Two Faces of the Liquid Ordered Phase
- 1.0554270 - ÚOCHB 2023 RIV US eng J - Článek v odborném periodiku
Schachter, Itay - Paananen, R. O. - Fábián, Balázs - Jurkiewicz, Piotr - Javanainen, Matti
The Two Faces of the Liquid Ordered Phase.
Journal of Physical Chemistry Letters. Roč. 13, č. 5 (2022), s. 1307-1313. ISSN 1948-7185
Grant CEP: GA ČR(CZ) GX19-26854X
Institucionální podpora: RVO:61388963 ; RVO:61388955
Klíčová slova: gel phase * phospholipid bilayers * molecular motion
Obor OECD: Physical chemistry; Physical chemistry (UFCH-W)
Impakt faktor: 5.7, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1021/acs.jpclett.1c03712
Coexisting liquid ordered (Lo) and liquid disordered (Ld) lipid phases in synthetic and plasma membrane-derived vesicles are commonly used to model the heterogeneity of biological membranes, including their putative ordered rafts. However, raft-associated proteins exclusively partition to the Ld and not the Lo phase in these model systems. We believe that the difference stems from the different microscopic structures of the lipid rafts at physiological temperature and the Lo phase studied at room temperature. To probe this structural diversity across temperatures, we performed atomistic molecular dynamics simulations, differential scanning calorimetry, and fluorescence spectroscopy on Lo phase membranes. Our results suggest that raft-associated proteins are excluded from the Lo phase at room temperature due to the presence of a stiff, hexagonally packed lipid structure. This structure melts upon heating, which could lead to the preferential solvation of proteins by order-preferring lipids. This structural transition is manifested as a subtle crossover in membrane properties, yet, both temperature regimes still fulfill the definition of the Lo phase. We postulate that in the compositionally complex plasma membrane and in vesicles derived therefrom, both molecular structures can be present depending on the local lipid composition. These structural differences must be taken into account when using synthetic or plasma membrane-derived vesicles as a model for cellular membrane heterogeneity below the physiological temperature.
Trvalý link: http://hdl.handle.net/11104/0328903
Vědecká data: Zenodo, Zenodo
Počet záznamů: 1