Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system

0553925 - BC 2022 RIV GB eng J - Článek v odborném periodiku
Horváthová, L. - Žárský, V. - Pánek, T. - Derelle, R. - Pyrih, Jan - Motyčková, A. - Klapsťová, V. - Vinopalova, M. - Marková, L. - Voleman, L. - Klimeš, V. - Petrů, M. - Vaitová, Z. - Čepička, I. - Hryzakova, K. - Harant, K. - Gray, M.W. - Chami, M. - Guilvout, I. - Francetic, O. - Lang, B. - Vlček, Čestmír - Tsaousis, A. - Eliaš, M. - Doležal, P.
Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system.
Nature Communications. Roč. 12, č. 1 (2021), č. článku 2947. E-ISSN 2041-1723
Grant CEP: GA MŠMT(CZ) EF16_019/0000759
Institucionální podpora: RVO:60077344 ; RVO:68378050
Klíčová slova: multiple sequence alignment * protein secretion * swiss-model * molecular architecture * structural insights * crystal-structure * atpase gspe * membrane * prediction * complex
Obor OECD: Microbiology; Microbiology (UMG-J)
Impakt faktor: 17.694, rok: 2021
Způsob publikování: Open access
https://www.nature.com/articles/s41467-021-23046-7

The type 2 secretion system (T2SS) is present in some Gram-negative eubacteria and used to secrete proteins across the outer membrane. Here we report that certain representative heteroloboseans, jakobids, malawimonads and hemimastigotes unexpectedly possess homologues of core T2SS components. We show that at least some of them are present in mitochondria, and their behaviour in biochemical assays is consistent with the presence of a mitochondrial T2SS-derived system (miT2SS). We additionally identified 23 protein families co-occurring with miT2SS in eukaryotes. Seven of these proteins could be directly linked to the core miT2SS by functional data and/or sequence features, whereas others may represent different parts of a broader functional pathway, possibly also involving the peroxisome. Its distribution in eukaryotes and phylogenetic evidence together indicate that the miT2SS-centred pathway is an ancestral eukaryotic trait. Our findings thus have direct implications for the functional properties of the early mitochondrion. Bacteria use the type 2 secretion system to secrete enzymes and toxins across the outer membrane to the environment. Here the authors analyse the T2SS pathway in three protist lineages and suggest that the early mitochondrion may have been capable of secreting proteins into the cytosol.
Trvalý link: http://hdl.handle.net/11104/0328602