Suppression of the non-linear background in a multimode fibre CARS endoscope

0553554 - ÚPT 2023 RIV US eng J - Článek v odborném periodiku
Pikálek, Tomáš - Stibůrek, Miroslav - Simpson, Stephen Hugh - Čižmár, Tomáš - Traegaardh, Johanna
Suppression of the non-linear background in a multimode fibre CARS endoscope.
Biomedical Optics Express. Roč. 13, č. 2 (2022), s. 862-874, č. článku 450375. ISSN 2156-7085
Grant CEP: GA MŠMT EF15_003/0000476; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) EF18_046/0016045
Výzkumná infrastruktura: Czech-BioImaging II - 90129
Institucionální podpora: RVO:68081731
Klíčová slova: resolution * microscopy * probe * myelin * light
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 3.4, rok: 2022
Způsob publikování: Open access
https://opg.optica.org/boe/fulltext.cfm?uri=boe-13-2-862&id=468619

Multimode fibres show great potential for use as miniature endoscopes for imaging deep in tissue with minimal damage. When used for coherent anti-Stokes Raman scattering (CARS) microscopy with femtosecond excitation sources, a high band-width probe is required to efficiently focus the broadband laser pulses at the sample plane. Although graded-index (GRIN) fibres have a large bandwidth, it is accompanied by a strong background signal from four-wave mixing and other non-linear processes occurring inside the fibre. We demonstrate that using a composite probe consisting of a GRIN fibre with a spliced on step-index fibre reduces the intensity of the non-linear background by more than one order of magnitude without significantly decreasing the focusing performance of the probe. Using this composite probe we acquire CARS images of biologically relevant tissue such as myelinated axons in the brain with good contrast.
Trvalý link: https://hdl.handle.net/11104/0333682