Tandem gene amplification restores photosystem II accumulation in cytochrome b(559) mutants of cyanobacteria

0553481 - MBÚ 2023 RIV GB eng J - Článek v odborném periodiku
Chiu, Y. - Fu, H. - Skotnicová, Petra - Lin, K. - Komenda, Josef - Chu, H.
Tandem gene amplification restores photosystem II accumulation in cytochrome b(559) mutants of cyanobacteria.
New Phytologist. Roč. 233, č. 2 (2022), s. 766-780. ISSN 0028-646X. E-ISSN 1469-8137
Grant CEP: GA ČR(CZ) GX19-29225X
GRANT EU: European Commission(CZ) 854126 - PhotoRedesign
Institucionální podpora: RVO:61388971
Klíčová slova: site-directed mutagenesis * pcc 6803 mutants * photosynthetic growth * electron flow * axial-ligands * synechocystis * complex * protein * repair * light * cyanobacterium * cytochrome b(559) * photosynthesis * photosystem II (PSII) * tandem gene amplification
Obor OECD: Microbiology
Impakt faktor: 9.4, rok: 2022
Způsob publikování: Open access
https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17785

Cytochrome (Cyt) b(559) is a key component of the photosystem II complex (PSII) that is essential for its proper functioning and assembly. Site-directed mutants of the model cyanobacterium Synechocystis sp. PCC6803 with mutated heme axial ligands of Cyt b(559) have little PSII and are therefore unable to grow photoautotrophically. Here we describe two types of Synechocystis autotrophic transformants that retained the same mutations in Cyt b(559) but are able to accumulate PSII and grow photoautotrophically. Whole-genome sequencing revealed that all of these autotrophic transformants carried a variable number of tandem repeats (from 5 to 15) of chromosomal segments containing the psbEFLJ operon. RNA-seq analysis showed greatly increased transcript levels of the psbEFLJ operon in these autotrophic transformants. Multiple copies of the psbEFLJ operon in these transformants were only maintained during autotrophic growth, while its copy numbers gradually decreased under photoheterotrophic conditions. Two-dimensional PAGE analysis of membrane proteins revealed a strong deficiency in PSII complexes in the Cyt b(559) mutants that was reversed in the autotrophic transformants. These results illustrate how tandem gene amplification restores PSII accumulation and photoautotrophic growth in Cyt b(559) mutants of cyanobacteria, and may serve as an important adaptive mechanism for cyanobacterial survival.
Trvalý link: http://hdl.handle.net/11104/0330387