Changes in glycosyl inositol phosphoceramides during the cell cycle of the red alga Galdieria sulphuraria

0553480 - MBÚ 2023 RIV GB eng J - Článek v odborném periodiku
Vítová, Milada - Čížková, Mária - Náhlík, Vít - Řezanka, Tomáš
Changes in glycosyl inositol phosphoceramides during the cell cycle of the red alga Galdieria sulphuraria.
Phytochemistry. Roč. 194, FEB 2022 (2022), č. článku 113025. ISSN 0031-9422. E-ISSN 1873-3700
GRANT EU: European Commission(CZ) ATCZ172 - Interreg V-A Austria – Czech Republic
Institucionální podpora: RVO:61388971
Klíčová slova: lipidomic analysis * sphingolipids * plants * identification * extraction * separation * ceramides * reveals * Galdieria sulphuraria * Galdieriaceae * Red alga * Glycosyl inositol phosphoceramides * Cell cycle * Liquid chromatography-electrospray mass * spectrometry
Obor OECD: Plant sciences, botany
Impakt faktor: 3.8, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0031942221003745?via%3Dihub

Sphingolipids are significant component of plant-cell plasma membranes, as well as algal membranes, and mediate various biological processes. One of these processes is the change in lipid content during the cell cycle. This change is key to understanding cell viability and proliferation. There are relatively few papers describing highly glycosylated glycosyl inositol phosphorylceramide (GIPC) due to problems associated with the extractability of GIPCs and their analysis, especially in algae. After alkaline hydrolysis of total lipids from the red alga Galdieria sulphuraria, GIPCs were measured by highresolution tandem mass spectrometry and fragmentation of precursor ions in an Orbitrap mass spectrometer in order to elucidate the structures of molecular species. Fragmentation experiments such as tandem mass spectrometry in the negative ion mode were performed to determine both the ceramide group and polar head structures. Measurement of mass spectra in the negative regime was possible because the phosphate group stabilizes negative molecular ions [M-H]-. Analysis: of GIPCs at various stages of the cell cycle provided information on their abundance. It was found that, depending on the phases of the cell cycle, in particular during division, the uptake of all three components of GIPC, i.e., long-chain amino alcohols, fatty acids, and polar heads, changes. Structural modifications of the polar headgroup significantly increased the number of molecular species. Analysis demonstrated a convex characteristic for molecular species with only one saccharide (hexose or hexuronic acid) as the polar head. For two carbohydrates, the course of Hex-HexA was linear, while for HexA-HexA it was concave. The same was true for GIPC with three and four monosaccharides.
Trvalý link: http://hdl.handle.net/11104/0330389