Comparative Aspects of Immunoglobulin Gene Rearrangement Arrays in Different Species

0553320 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
Šinkora, Marek - Štěpánová, Kateřina - Butler, J. E. - Šinkora jr., Marek - Šinkora, Šimon - Šinkorová, Jana
Comparative Aspects of Immunoglobulin Gene Rearrangement Arrays in Different Species.
Frontiers in Immunology. Roč. 13, February 2022 (2022), č. článku 823145. ISSN 1664-3224. E-ISSN 1664-3224
Grant CEP: GA ČR(CZ) GA19-01504S; GA ČR(CZ) GA20-03282S
Institucionální podpora: RVO:61388971
Klíčová slova: B cell development * B cell receptors * cell differentiation * immunoglobulin heavy and light chains * gene rearrangement
Obor OECD: Immunology
Impakt faktor: 7.3, rok: 2022
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fimmu.2022.823145/full

Studies in humans and mice indicate the critical role of the surrogate light chain in the
selection of the productive immunoglobulin repertoire during B cell development.
However, subsequent studies using mutant mice have also demonstrated that
alternative pathways are allowed. Our recent investigation has shown that some
species, such as pig, physiologically use preferential rearrangement of authentic light
chains, and become independent of surrogate light chains. Here we summarize the
findings from swine and compare them with results in other species. In both groups, allelic
and isotypic exclusions remain intact, so the different processes do not alter the paradigm
of B-cell monospecificity. Both groups also retained some other essential processes, such
as segregated and sequential rearrangement of heavy and light chain loci, preferential
rearrangement of light chain kappa before lambda, and functional k-deleting element
recombination. On the other hand, the respective order of heavy and light chains
rearrangement may vary, and rearrangement of the light chain kappa and lambda on
different chromosomes may occur independently. Studies have also confirmed that the
surrogate light chain is not required for the selection of the productive repertoire of heavy
chains and can be substituted by authentic light chains. These findings are important for
understanding evolutional approaches, redundancy and efficiency of B-cell generation,
dependencies on other regulatory factors, and strategies for constructing therapeutic
antibodies in unrelated species. The results may also be important for explaining
interspecies differences in the proportional use of light chains and for the understanding
of divergences in rearrangement processes. Therefore, the division into two groups may
not be definitive and there may be more groups of intermediate species.
Trvalý link: http://hdl.handle.net/11104/0328299