Host tropism determination by convergent evolution of immunological evasion in the Lyme disease system

0553232 - BC 2022 RIV US eng J - Článek v odborném periodiku
Hart, T. - Dupuis, A. P. - Tufts, D. - Blom, A. - Starkey, S. - Rego, Ryan O. M. - Ram, S. - Kraiczy, P. - Kramer, L. - Diuk-Wasser, M. - Kolokotronis, S. - Lin, Y.
Host tropism determination by convergent evolution of immunological evasion in the Lyme disease system.
PLoS Pathogens. Roč. 17, č. 7 (2021), č. článku e1009801. ISSN 1553-7366. E-ISSN 1553-7374
Grant CEP: GA ČR(CZ) GA17-21244S
Institucionální podpora: RVO:60077344
Klíčová slova: acquiring surface-proteins * burgdorferi sensu-lato * tick salivary protein * regulator factor-h * borrelia-burgdorferi * functional-characterization * complement resistance * ixodes-scapularis * avian reservoir * plasmid content
Obor OECD: Microbiology
Impakt faktor: 7.464, rok: 2021
Způsob publikování: Open access
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009801

Pathogens possess the ability to adapt and survive in some host species but not in others-an ecological trait known as host tropism. Transmitted through ticks and carried mainly by mammals and birds, the Lyme disease (LD) bacterium is a well-suited model to study such tropism. Three main causative agents of LD, Borrelia burgdorferi, B. afzelii, and B. garinii, vary in host ranges through mechanisms eluding characterization. By feeding ticks infected with different Borrelia species, utilizing feeding chambers and live mice and quail, we found species-level differences in bacterial transmission. These differences localize on the tick blood meal, and specifically complement, a defense in vertebrate blood, and a polymorphic bacterial protein, CspA, which inactivates complement by binding to a host complement inhibitor, Factor H (FH). CspA selectively confers bacterial transmission to vertebrates that produce FH capable of allele-specific recognition. CspA is the only member of the Pfam54 gene family to exhibit host-specific FH-binding. Phylogenetic analyses revealed convergent evolution as the driver of such uniqueness, and that FH-binding likely emerged during the last glacial maximum. Our results identify a determinant of host tropism in Lyme disease infection, thus defining an evolutionary mechanism that shapes host-pathogen associations.
Trvalý link: http://hdl.handle.net/11104/0328224