In Situ monitoring of rolling circle amplification on a solid support by surface plasmon resonance and optical waveguide spectroscopy

0552380 - FZÚ 2022 RIV US eng J - Článek v odborném periodiku
Lechner, B. - Hageneder, S. - Schmidt, K. - Kreuzer, M. - Conzemius, R. - Reimhult, E. - Barisi, I. - Dostálek, Jakub
In Situ monitoring of rolling circle amplification on a solid support by surface plasmon resonance and optical waveguide spectroscopy.
ACS Applied Materials and Interfaces. Roč. 13, č. 27 (2021), s. 32352-32362. ISSN 1944-8244. E-ISSN 1944-8252
Grant CEP: GA MŠMT(CZ) EF18_053/0016627
Grant ostatní: OP VVV - Mobility FZU 2(XE) CZ.02.2.69/0.0/0.0/18_053/0016627
Institucionální podpora: RVO:68378271
Klíčová slova: rolling circle amplification * DNA * polyelectrolyte brushes * surface plasmon resonance * optical waveguide spectroscopy * surface plasmon-enhanced fluorescence * biointerfaces
Obor OECD: Biochemical research methods
Impakt faktor: 10.383, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1021/acsami.1c03715

The growth of surface-attached single-stranded deoxyribonucleic acid (ssDNA) chains is monitored in situ using an evanescent wave optical biosensor that combines surface plasmon resonance (SPR) and optical waveguide spectroscopy (OWS). The “grafting-from” growth of ssDNA chains is facilitated by rolling circle amplification (RCA), and the gradual prolongation of ssDNA chains anchored to a gold sensor surface is optically tracked in time. At a sufficient density of the polymer chains, the ssDNA takes on a brush architecture with a thickness exceeding 10 μm, supporting a spectrum of guided optical waves traveling along the metallic sensor surface. The simultaneous probing of this interface with the confined optical field of surface plasmons and additional more delocalized dielectric optical waveguide modes enables accurate in situ measurement of the ssDNA brush thickness, polymer volume content, and density gradients.
Trvalý link: http://hdl.handle.net/11104/0327526