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Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

  1. 1.
    0552209 - BC 2022 RIV CH eng J - Článek v odborném periodiku
    Beliavskaia, A. - Hönig, Václav - Erhart, Jan - Vyhlídalová, Tereza - Palus, Martin - Černý, J. - Kozlová, I. - Růžek, Daniel - Palomar, A. - Bell-Sakyi, L.
    Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks.
    Frontiers in Veterinary Science. Roč. 8, MAR 26 2021 (2021), č. článku 659786. E-ISSN 2297-1769
    Grant CEP: GA ČR(CZ) GA20-14325S; GA ČR(CZ) GA20-30500S; GA MZe(CZ) QK1920258
    Institucionální podpora: RVO:60077344
    Klíčová slova: tick cell line * endosymbiont * Spiroplasma * tick colony * Ixodes persulcatus
    Obor OECD: Microbiology
    Impakt faktor: 3.471, rok: 2021
    Způsob publikování: Open access
    https://www.frontiersin.org/articles/10.3389/fvets.2021.659786/full

    Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes, 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.
    Trvalý link: http://hdl.handle.net/11104/0327346

     
     
Počet záznamů: 1  

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