Evolutionary Analysis of Cystatins of Early-Emerging Metazoans Reveals a Novel Subtype in Parasitic Cnidarians

0552127 - BC 2022 RIV CH eng J - Článek v odborném periodiku
Bartošová-Sojková, Pavla - Kyslík, Jiří - Alama-Bermejo, Gema - Hartigan, A. - Atkinson, S. - Bartholomew, J. - Picard-Sanchez, Maria Amparo - Palenzuela, O. - Faber, M. - Holland, J. - Holzer, Astrid S.
Evolutionary Analysis of Cystatins of Early-Emerging Metazoans Reveals a Novel Subtype in Parasitic Cnidarians.
Biology. Roč. 10, č. 2 (2021), č. článku 110. E-ISSN 2079-7737
Grant CEP: GA MŠMT(CZ) LTAUSA17201; GA ČR(CZ) GX19-28399X; GA ČR(CZ) GA21-16565S
GRANT EU: European Commission(XE) 634429 - ParaFishControl
Institucionální podpora: RVO:60077344
Klíčová slova: cysteine protease inhibitor * stefin * signal peptide * parasite * phylogenetic analysis * diversification * protein structure
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.168, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/2079-7737/10/2/110

The evolutionary aspects of cystatins are greatly underexplored in early-emerging metazoans. Thus, we surveyed the gene organization, protein architecture, and phylogeny of cystatin homologues mined from 110 genomes and the transcriptomes of 58 basal metazoan species, encompassing free-living and parasite taxa of Porifera, Placozoa, Cnidaria (including Myxozoa), and Ctenophora. We found that the cystatin gene repertoire significantly differs among phyla, with stefins present in most of the investigated lineages but with type 2 cystatins missing in several basal metazoan groups. Similar to liver and intestinal flukes, myxozoan parasites possess atypical stefins with chimeric structure that combine motifs of classical stefins and type 2 cystatins. Other early metazoan taxa regardless of lifestyle have only the classical representation of cystatins and lack multi-domain ones. Our comprehensive phylogenetic analyses revealed that stefins and type 2 cystatins clustered into taxonomically defined clades with multiple independent paralogous groups, which probably arose due to gene duplications. The stefin clade split between the subclades of classical stefins and the atypical stefins of myxozoans and flukes. Atypical stefins represent key evolutionary innovations of the two parasite groups for which their origin might have been linked with ancestral gene chimerization, obligate parasitism, life cycle complexity, genome reduction, and host immunity.
Trvalý link: http://hdl.handle.net/11104/0327278