M13 Phages Uptake of Gold Nanoparticles for Radio- and Thermal-Therapy and Contrast Imaging Improvement

0551777 - ÚJF 2022 RIV CH eng J - Článek v odborném periodiku
Torrisi, L. - Silipigni, L. - Kováčik, L. - Lavrentiev, Vasyl - Cutroneo, Mariapompea - Torrisi, A. - De Plano, L. M. - Franco, D. - Guglielmino, S.
M13 Phages Uptake of Gold Nanoparticles for Radio- and Thermal-Therapy and Contrast Imaging Improvement.
Applied Sciences-Basel. Roč. 11, č. 23 (2021), č. článku 11391. E-ISSN 2076-3417
Grant CEP: GA MŠMT EF16_013/0001812
Výzkumná infrastruktura: CANAM II - 90056
Institucionální podpora: RVO:61389005
Klíčová slova: gold nanoparticles * hyperthermia * M13 phages * radiotherapy
Obor OECD: Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)
Impakt faktor: 2.838, rok: 2021
Způsob publikování: Open access
https://doi.org/10.3390/app112311391

The presented work deals with the uptake of gold nanoparticles (Au NPs) by M13 phages in solutions. In particular, the Au NPs uptake modalities and their localization in the filamentous phages are evaluated and measured. Gold spherical nanoparticles (with an average diameter of the order of 10 nm) are obtained by laser ablation in water with a sodium citrated surfactant. The interest of such application comes from the possibility to employ living biological structures to transport heavy metallic nanoparticles inside cells of tumoral tissues. Indeed, phages have the capability to introduce Au NPs in the proximity to the cell nucleus, increasing the efficiency of DNA destruction in the tumoral cells by employing low doses of ionizing radiation during radiotherapy and hyperthermia treatments. Several analyses and microscopy characterizations of the prepared phages samples embedding gold nanoparticles are presented, demonstrating that the presence of Au NPs increases the phages imaging contrast.
Trvalý link: http://hdl.handle.net/11104/0326995