Počet záznamů: 1
Fast Leaps between Millisecond Confinements Govern Ase1 Diffusion along Microtubules
- 1.0551738 - ÚFE 2022 RIV DE eng J - Článek v odborném periodiku
Bujak, Lukasz - Holanová, Kristýna - Marín, Antonio García - Henrichs, Verena - Barvík, I. - Braun, Marcus - Lánský, Zdeněk - Piliarik, Marek
Fast Leaps between Millisecond Confinements Govern Ase1 Diffusion along Microtubules.
Small Methods. Roč. 5, č. 10 (2021), č. článku 2100370. ISSN 2366-9608. E-ISSN 2366-9608
Grant CEP: GA MŠMT(CZ) LL1602; GA ČR(CZ) GX19-27477X
Institucionální podpora: RVO:67985882 ; RVO:86652036
Klíčová slova: Asel * Coarse-grain model * Interferometric scattering microscopy * Energy landscape
Obor OECD: Optics (including laser optics and quantum optics); Biophysics (BTO-N)
Impakt faktor: 15.367, rok: 2021
Způsob publikování: Open access
https://doi.org/10.1002/smtd.202100370
Diffusion is the most fundamental mode of protein translocation within cells. Confined diffusion of proteins along the electrostatic potential constituted by the surface of microtubules, although modeled meticulously in molecular dynamics simulations, has not been experimentally observed in real-time. Here, interferometric scattering microscopy is used to directly visualize the movement of the microtubule-associated protein Ase1 along the microtubule surface at nanometer and microsecond resolution. Millisecond confinements of Ase1 and fast leaps between these positions of dwelling preferentially occurring along the microtubule protofilaments are resolved, revealing Ase1's mode of diffusive translocation along the microtubule's periodic surface. The derived interaction potential closely matches the tubulin-dimer periodicity and the distribution of the electrostatic potential on the microtubule lattice. It is anticipated that mapping the interaction landscapes for different proteins on microtubules, finding plausible energetic barriers of different positioning and heights, can provide valuable insights into regulating the dynamics of essential cytoskeletal processes, such as intracellular cargo trafficking, cell division, and morphogenesis, all of which rely on diffusive translocation of proteins along microtubules
Trvalý link: http://hdl.handle.net/11104/0326960
Počet záznamů: 1