EMC is required for biogenesis of Xport-A, an essential chaperone of Rhodopsin-1 and the TRP channel

0551576 - ÚOCHB 2023 RIV US eng J - Článek v odborném periodiku
Gaspar, C. J. - Vieira, L. C. - Santos, C. C. - Christianson, J. C. - Jakubec, Dávid - Stříšovský, Kvido - Adrain, C. - Domingos, P. M.
EMC is required for biogenesis of Xport-A, an essential chaperone of Rhodopsin-1 and the TRP channel.
Embo Reports. Roč. 23, č. 1 (2022), č. článku e53210. ISSN 1469-221X. E-ISSN 1469-3178
Institucionální podpora: RVO:61388963
Klíčová slova: ER membrane protein complex * Rh1 * tail anchored proteins * TRP * Xport-A
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 7.7, rok: 2022
Způsob publikování: Open access
https://doi.org/10.15252/embr.202153210

The ER membrane protein complex (EMC) is required for the biogenesis of a subset of tail anchored (TA) and polytopic membrane proteins, including Rhodopsin-1 (Rh1) and the TRP channel. To understand the physiological implications of EMC-dependent membrane protein biogenesis, we perform a bioinformatic identification of Drosophila TA proteins. From 254 predicted TA proteins, screening in larval eye discs identified two proteins that require EMC for their biogenesis: fan and Xport-A. Fan is required for male fertility in Drosophila and we show that EMC is also required for this process. Xport-A is essential for the biogenesis of both Rh1 and TRP, raising the possibility that disruption of Rh1 and TRP biogenesis in EMC mutants is secondary to the Xport-A defect. We show that EMC is required for Xport-A TMD membrane insertion and that EMC-independent Xport-A mutants rescue Rh1 and TRP biogenesis in EMC mutants. Finally, our work also reveals a role for Xport-A in a glycosylation-dependent triage mechanism during Rh1 biogenesis in the endoplasmic reticulum.
Trvalý link: http://hdl.handle.net/11104/0326836


Vědecká data: GitHub