Počet záznamů: 1  

Assessment of Possible Contributions of Hyaluronan and Proteoglycan Binding Link Protein 4 to Differential Perineuronal Net Formation at the Calyx of Held

  1. 1.
    0551546 - ÚEM 2022 RIV CH eng J - Článek v odborném periodiku
    Nojima, K. - Miyazaki, H. - Hori, T. - Vargová, Lýdia - Oohashi, T.
    Assessment of Possible Contributions of Hyaluronan and Proteoglycan Binding Link Protein 4 to Differential Perineuronal Net Formation at the Calyx of Held.
    Frontiers in Cell and Developmental Biology. Roč. 9, sep. (2021), č. článku 730550. ISSN 2296-634X. E-ISSN 2296-634X
    Institucionální podpora: RVO:68378041
    Klíčová slova: perineuronal net * chondroitin sulfate proteoglycan * calyx of Held * hyaluronan and proteoglycan binding link protein 4 * synapse * in situ proximity ligation assay
    Obor OECD: Neurosciences (including psychophysiology
    Impakt faktor: 6.081, rok: 2021
    Způsob publikování: Open access
    https://www.frontiersin.org/articles/10.3389/fcell.2021.730550/full

    The calyx of Held is a giant nerve terminal mediating high-frequency excitatory input to principal cells of the medial nucleus of the trapezoid body (MNTB). MNTB principal neurons are enwrapped by densely organized extracellular matrix structures, known as perineuronal nets (PNNs). Emerging evidence indicates the importance of PNNs in synaptic transmission at the calyx of Held. Previously, a unique differential expression of aggrecan and brevican has been reported at this calyceal synapse. However, the role of hyaluronan and proteoglycan binding link proteins (HAPLNs) in PNN formation and synaptic transmission at this synapse remains elusive. This study aimed to assess immunohistochemical evidence for the effect of HAPLN4 on differential PNN formation at the calyx of Held. Genetic deletion of Hapln4 exhibited a clear ectopic shift of brevican localization from the perisynaptic space between the calyx of Held terminals and principal neurons to the neuropil surrounding the whole calyx of Held terminals. In contrast, aggrecan expression showed a consistent localization at the surrounding neuropil, together with HAPLN1 and tenascin-R, in both gene knockout (KO) and wild-type (WT) mice. An in situ proximity ligation assay demonstrated the molecular association of brevican with HAPLN4 in WT and HAPLN1 in gene KO mice. Further elucidation of the roles of HAPLN4 may highlight the developmental and physiological importance of PNN formation in the calyx of Held.
    Trvalý link: http://hdl.handle.net/11104/0326820

     
     
Počet záznamů: 1  

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