Tumor vessel co-option probed by single-cell analysis

0550598 - BTÚ 2022 RIV GB eng J - Článek v odborném periodiku
Teuwen, L.-A. - De Rooij, L. P. M. H. - Cuypers, A. - Rohlenová, Kateřina - Dumas, S. J. - Garcia-Caballero, M. - Meta, E. - Amersfoort, J. - Taverna, F. - Becker, L. M. - Veiga, N. - Cantelmo, A. R. - Geldhof, V. - Conchinha, N. - Kalucka, J. - Treps, L. - Conradi, L.-Ch. - Khan, S. - Karakach, T. K. - Soenen, S. - Vinckier, S. - Schoonjans, L. - Eelen, G. - Van Laere, S. - Dewerchin, M. - Dirix, L. - Mazzone, M. - Luo, Y. - Vermeulen, P. - Carmeliet, P.
Tumor vessel co-option probed by single-cell analysis.
Cell Reports. Roč. 35, č. 11 (2021), č. článku 109253. ISSN 2211-1247. E-ISSN 2211-1247
Institucionální podpora: RVO:86652036
Klíčová slova: anti-angiogenic therapy * epithelium-derived factor * cancer liver metastases * smooth-muscle-cells * gene-expression * pdgf-b
Obor OECD: Cell biology
Impakt faktor: 9.995, rok: 2021
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S2211124721006185?via%3Dihub

Tumor vessel co-option is poorly understood, yet it is a resistance mechanism against anti-angiogenic therapy (AAT). The heterogeneity of co-opted endothelial cells (ECs) and pericytes, co-opting cancer andmyeloid cells in tumors growing via vessel co-option, has not been investigated at the single-cell level. Here, we use a murine AAT-resistant lung tumor model, in which VEGF-targeting induces vessel co-option for continued growth. Single-cell RNA sequencing (scRNA-seq) of 31,964 cells reveals, unexpectedly, a largely similar transcriptome of co-opted tumor ECs (TECs) and pericytes as their healthy counterparts. Notably, we identify cell types that might contribute to vessel co-option, i.e., an invasive cancer-cell subtype, possibly assisted by a matrix-remodeling macrophage population, and another M1-like macrophage subtype, possibly involved in keeping or rendering vascular cells quiescent.
Trvalý link: http://hdl.handle.net/11104/0326792