Počet záznamů: 1  

alpha V Integrin Expression and Localization in Male Germ Cells

  1. 1.
    0550583 - BTÚ 2022 RIV CH eng J - Článek v odborném periodiku
    Páleníková, Veronika - Frolíková, Michaela - Valášková, Eliška - Postlerová, Pavla - Komrsková, Kateřina
    alpha V Integrin Expression and Localization in Male Germ Cells.
    International Journal of Molecular Sciences. Roč. 22, č. 17 (2021), č. článku 9525. E-ISSN 1422-0067
    Grant CEP: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) ED1.1.00/02.0109
    Institucionální podpora: RVO:86652036
    Klíčová slova: alpha V integrins * male germ cell * mouse
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 6.208, rok: 2021
    Způsob publikování: Open access
    https://www.mdpi.com/1422-0067/22/17/9525

    Integrins are transmembrane receptors that facilitate cell adhesion and cell-extracellular matrix communication. They are involved in the sperm maturation including capacitation and gamete interaction, resulting in successful fertilization. alpha V integrin belongs to the integrin glycoprotein superfamily, and it is indispensable for physiological spermiogenesis and testosterone production. We targeted the gene and protein expression of the alpha V integrin subunit and described its membrane localization in sperm. Firstly, in mouse, we traced alpha V integrin gene expression during spermatogenesis in testicular fraction separated by elutriation, and we detected gene activity in spermatogonia, spermatocytes, and round spermatids. Secondly, we specified alpha V integrin membrane localization in acrosome-intact and acrosome-reacted sperm and compared its pattern between mouse, pig, and human. Using immunodetection and structured illumination microscopy (SIM), the alpha V integrin localization was confined to the plasma membrane covering the acrosomal cap area and also to the inner acrosomal membrane of acrosome-intact sperm of all selected species. During the acrosome reaction, which was induced on capacitated sperm, the alpha V integrin relocated and was detected over the whole sperm head. Knowledge of the integrin pattern in mature sperm prepares the ground for further investigation into the pathologies and related fertility issues in human medicine and veterinary science.
    Trvalý link: http://hdl.handle.net/11104/0328118

     
     
Počet záznamů: 1  

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