Insect body defence reactions against bee venom: Do adipokinetic hormones play a role?

0550049 - BC 2023 RIV CH eng J - Článek v odborném periodiku
Bodláková, Karolína - Černý, Jan - Štěrbová, Helena - Guráň, R. - Zítka, O. - Kodrík, Dalibor
Insect body defence reactions against bee venom: Do adipokinetic hormones play a role?
Toxins. Roč. 14, č. 1 (2022), č. článku 11. ISSN 2072-6651. E-ISSN 2072-6651
Institucionální podpora: RVO:60077344
Klíčová slova: adipokinetic hormone * American cockroach * dopamine
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.2, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2072-6651/14/1/11/pdf

Bees originally developed their stinging apparatus and venom against members of their own species from other hives or against predatory insects. Nevertheless, the biological and biochemical response of arthropods to bee venom is not well studied. Thus, in this study, the physiological responses of a model insect species (American cockroach, Periplaneta americana) to honeybee venom were investigated. Bee venom toxins elicited severe stress (LD50 = 1.063 uL venom) resulting in a significant increase in adipokinetic hormones (AKHs) in the cockroach central nervous system and haemolymph. Venom treatment induced a large destruction of muscle cell ultrastructure, especially myofibrils and sarcomeres. Interestingly, co-application of venom with cockroach Peram-CAH-II AKH eliminated this effect. Envenomation modulated the levels of carbohydrates, lipids, and proteins in the haemolymph and the activity of digestive amylases, lipases, and proteases in the midgut. Bee venom significantly reduced vitellogenin levels in females. Dopamine and glutathione (GSH and GSSG) insignificantly increased after venom treatment. However, dopamine levels significantly increased after Peram-CAH-II application and after co-application with bee venom, while GSH and GSSG levels immediately increased after co-application. The results suggest a general reaction of the cockroach body to bee venom and at least a partial involvement of AKHs.
Trvalý link: https://hdl.handle.net/11104/0338968