TRPM7 N-terminal region forms complexes with calcium binding proteins CaM and S100A1

0549345 - ÚOCHB 2022 RIV GB eng J - Článek v odborném periodiku
Boušová, Kristýna - Zouharová, Monika - Herman, P. - Vetýšková, Veronika - Jirásková, Kateřina - Vondrášek, Jiří
TRPM7 N-terminal region forms complexes with calcium binding proteins CaM and S100A1.
Heliyon. Roč. 7, č. 12 (2021), č. článku e08490. ISSN 2405-8440. E-ISSN 2405-8440
Institucionální podpora: RVO:61388963
Klíčová slova: TRPM7 * binding region * CaM * S100A1 * calcium * fluorescence anisotropy
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.776, rok: 2021
Způsob publikování: Open access
https://doi.org/10.1016/j.heliyon.2021.e08490

Transient receptor potential melastatin 7 (TRPM7) represents melastatin TRP channel with two significant functions, cation permeability and kinase activity. TRPM7 is widely expressed among tissues and is therefore involved in a variety of cellular functions representing mainly Mg2+ homeostasis, cellular Ca2+ flickering, and the regulation of DNA transcription by a cleaved kinase domain translocated to the nucleus. TRPM7 participates in several important biological processes in the nervous and cardiovascular systems. Together with the necessary function of the TRPM7 in these tissues and its recently analyzed overall structure, this channel requires further studies leading to the development of potential therapeutic targets. Here we present the first study investigating the N-termini of TRPM7 with binding regions for important intracellular modulators calmodulin (CaM) and calcium-binding protein S1 (S100A1) using in vitro and in silico approaches. Molecular simulations of the discovered complexes reveal their potential binding interfaces with common interaction patterns and the important role of basic residues present in the N-terminal binding region of TRPM.
Trvalý link: http://hdl.handle.net/11104/0325358