Critical Reviews and Perspectives beta-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck

0549270 - MBÚ 2022 RIV GB eng J - Článek v odborném periodiku
Wiedermannova, J. - Krásný, Libor
Critical Reviews and Perspectives beta-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck.
Nucleic Acids Research. Roč. 49, č. 18 (2021), s. 10221-10234. ISSN 0305-1048. E-ISSN 1362-4962
Grant CEP: GA ČR(CZ) GA19-12956S
Institucionální podpora: RVO:61388971
Klíčová slova: pre-messenger-rna * promotes transcription termination * escherichia-coli * elongation-factor * ii termination * premature termination * archaeal cleavage * bacterial homolog * crystal-structure * molecular-basis
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 19.160, rok: 2021
Způsob publikování: Open access
https://academic.oup.com/nar/article/49/18/10221/6374178

During the first step of gene expression, RNA polymerase (RNAP) engages DNA to transcribe RNA, forming highly stable complexes. These complexes need to be dissociated at the end of transcription units or when RNAP stalls during elongation and becomes an obstacle ('sitting duck') to further transcription or replication. In this review, we first outline the mechanisms involved in these processes. Then, we explore in detail the torpedo mechanism whereby a 5'-3' RNA exonuclease (torpedo) latches itself onto the 5' end of RNA protruding from RNAP, degrades it and upon contact with RNAP, induces dissociation of the complex. This mechanism, originally described in Eukaryotes and executed by Xrn-type 5'-3' exonucleases, was recently found in Bacteria and Archaea, mediated by beta-CASP family exonucleases. We discuss the mechanistic aspects of this process across the three kingdoms of life and conclude that 5'-3' exoribonucleases (beta-CASP and Xrn families) involved in the ancient torpedo mechanism have emerged at least twice during evolution.
Trvalý link: http://hdl.handle.net/11104/0325298