Comparison of the Covalent Laccase Immobilization at Amino- and Carboxylfunctionalized Mesoporous Silica, Glassy Carbon, and Graphite Powders using Different Coupling Agents for Optimal Biosensor Preparation

0549133 - ÚFCH JH 2024 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Tvorynska, Sofiia - Barek, J. - Josypčuk, Bohdan
Comparison of the Covalent Laccase Immobilization at Amino- and Carboxylfunctionalized Mesoporous Silica, Glassy Carbon, and Graphite Powders using Different Coupling Agents for Optimal Biosensor Preparation.
Sborník přednášek mezinárodní odborné konference XL. Moderní Elektrochemické Metody. Ústí nad Labem: Best Servis, 2021 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), s. 248-252. ISBN 978-80-905221-8-3.
[Moderní Elektrochemické Metody /40./. Jetřichovice (CZ), 08.11.2021-12.11.2021]
Grant CEP: GA ČR(CZ) GA20-01589S; GA ČR(CZ) GA20-07350S
Institucionální podpora: RVO:61388955
Klíčová slova: Covalent enzyme immobilization * Support surface functionalization * Laccase
Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

In order to find the most suitable immobilization protocol, a comparison of three strategies
based on the application of –NH2 and –COOH functionalized supports with the different
activation agents (glutaraldehyde and carbodiimide) have been conducted for the covalent
enzyme (laccase) attachment. Two kinds of the supports, namely mesoporous silica (SBA−15,
MCM−41) and carbonaceous (glassy carbon, graphite) powders, have been used. It was found
that a biosensor consisted of tubular detector of silver solid amalgam as a working electrode
and the enzymatic mini-reactor with laccase covalently attached to glutaraldehyde activated
–NH2 functionalized MCM−41 shows the best results regarding sensitivity and stability for
dopamine detection.
Trvalý link: http://hdl.handle.net/11104/0325150