Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum

0548764 - BC 2022 RIV GB eng J - Článek v odborném periodiku
Škodová-Sveráková, Ingrid - Záhonová, Kristýna - Juricová, Valéria - Danchenko, M. - Moos, Martin - Baráth, P. - Prokopchuk, Galina - Butenko, Anzhelika - Lukáčová, V. - Kohútová, L. - Bučková, B. - Horák, Aleš - Faktorová, Drahomíra - Horváth, A. - Šimek, Petr - Lukeš, Julius
Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum.
BMC BIOLOGY. Roč. 19, č. 1 (2021), č. článku 251. E-ISSN 1741-7007
Grant CEP: GA ČR(CZ) GA18-23787S; GA ČR(CZ) GA17-22276S; GA ČR(CZ) GA18-15962S; GA MŠMT(CZ) LL1601; GA MŠMT(CZ) EF16_019/0000759
Grant ostatní: Gordon and Betty Moore Foundation(US) #9354
Program: Science
Institucionální podpora: RVO:60077344
Klíčová slova: Diplonema * metabolism * multiomics
Obor OECD: Microbiology
Impakt faktor: 7.364, rok: 2021
Způsob publikování: Open access
https://bmcbiol.biomedcentral.com/track/pdf/10.1186/s12915-021-01186-y.pdf

Background
The phylum Euglenozoa is a group of flagellated protists comprising the diplonemids, euglenids, symbiontids, and kinetoplastids. The diplonemids are highly abundant and speciose, and recent tools have rendered the best studied representative, Diplonema papillatum, genetically tractable. However, despite the high diversity of diplonemids, their lifestyles, ecological functions, and even primary energy source are mostly unknown.
Results
We designed a metabolic map of D. papillatum cellular bioenergetic pathways based on the alterations of transcriptomic, proteomic, and metabolomic profiles obtained from cells grown under different conditions. Comparative analysis in the nutrient-rich and nutrient-poor media, as well as the absence and presence of oxygen, revealed its capacity for extensive metabolic reprogramming that occurs predominantly on the proteomic rather than the transcriptomic level. D. papillatum is equipped with fundamental metabolic routes such as glycolysis, gluconeogenesis, TCA cycle, pentose phosphate pathway, respiratory complexes, β-oxidation, and synthesis of fatty acids. Gluconeogenesis is uniquely dominant over glycolysis under all surveyed conditions, while the TCA cycle represents an eclectic combination of standard and unusual enzymes.
Conclusions
The identification of conventional anaerobic enzymes reflects the ability of this protist to survive in low-oxygen environments. Furthermore, its metabolism quickly reacts to restricted carbon availability, suggesting a high metabolic flexibility of diplonemids, which is further reflected in cell morphology and motility, correlating well with their extreme ecological valence.
Trvalý link: http://hdl.handle.net/11104/0326650