Interactions between zinc and Phomopsis longicolla infection in roots of Glycine max

0548246 - BC 2022 RIV GB eng J - Článek v odborném periodiku
Morina, Filis - Mijovilovich, Ana - Koloniuk, Igor - Pěnčík, Aleš - Grúz, Jiří - Novák, Ondřej - Küpper, Hendrik
Interactions between zinc and Phomopsis longicolla infection in roots of Glycine max.
Journal of Experimental Botany. Roč. 72, č. 8 (2021), s. 3320-3336. ISSN 0022-0957. E-ISSN 1460-2431
Grant CEP: GA MŠMT EF15_003/0000336
Institucionální podpora: RVO:60077344 ; RVO:61389030
Klíčová slova: Jasmonates * metal tissue distribution * micro-XRF * phenolics * Phomopsis longicolla * salicylic acid * soybean * root * zinc
Obor OECD: Biochemistry and molecular biology; Biochemistry and molecular biology (UEB-Q)
Impakt faktor: 7.378, rok: 2021
Způsob publikování: Open access
https://academic.oup.com/jxb/article/72/8/3320/6129311

Phomopsis. longicolla is a hemibiotrophic fungus causing significant soybean yield loss worldwide. To reveal the role of zinc in plant-pathogen interactions, soybean seedlings were grown hydroponically with a range of Zn concentrations, 0.06 mu M (deficient, Zn0), 0.4 mu M (optimal growth), 1.5 mu M, 4 mu M, 12 mu M, and toxic 38 mu M, and were subsequently inoculated with P. longicolla via the roots. In vivo analysis of metal distribution in tissues by micro-X-ray fluorescence showed local Zn mobilization in the root maturation zone in all treatments. Decreased root and pod biomass, and photosynthetic performance in infected plants treated with 0.4 mu M Zn were accompanied with accumulation of Zn, jasmonoyl-L-isoleucine (JA-Ile), jasmonic acid, and cell wall-bound syringic acid ((cw)SyA) in roots. Zn concentration in roots of infected plants treated with 1.5 mu M Zn was seven-fold higher than in the 0.4 mu M Zn treatment, which together with accumulation of JA-Ile, (cw)SyA, cell wall-bound vanilic acid and leaf jasmonates contributed to maintaining photosynthesis and pod biomass. Host-pathogen nutrient competition and phenolics accumulation limited the infection in Zn-deficient plants. The low infection rate in Zn 4 mu M-treated roots correlated with salicylic and 4-hydroxybenzoic acid, and cell wall-bound p-coumaric acid accumulation. Zn toxicity promoted pathogen invasion and depleted cell wall-bound phenolics. The results show that manipulation of Zn availability improves soybean resistance to P. longicolla by stimulating phenolics biosynthesis and stress-inducible phytohormones.
Trvalý link: http://hdl.handle.net/11104/0329097