Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements.

0547305 - ÚCHP 2022 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Zajíc, J. - Ripp, S. - Trögl, J. - Kuncová, Gabriela - Pospíšilová, M.
Whole-cell detectors of contaminants constructed by immobilization of bioreporters in form of biofilm on special optical fiber elements.
International Conference on Sensors and Nanotechnology IEEE /1./. New York: Institute of Electrical and Electronics Engineers Inc., 2019, s. 185-188. ISBN 978-1-5386-5619-8.
[IEEE International Conference on Sensors and Nanotechnology. Penang (MY), 24.07.2019-25.07.2019]
Institucionální podpora: RVO:67985858
Klíčová slova: biosensor * bioluminescent bioreporter * optical fiber sensor
Obor OECD: Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
https://ieeexplore.ieee.org/document/8940080

A whole-cell optical fiber sensor of toluene was prepared by the adsorption of Pseudomonas putida TVA8, the bacterial strain producing light in contact with toluene, in the form of biofilm. Bioreporter immobilization was facilitated by (3-Aminopropyl)triethoxysilane (APTES) treatment on the wider end of tapered quartz optical fiber element (OFE). Repeatability of preparation and response of the active part of the biosensor was demonstrated in five trials with different OFE geometries in which the bioluminescence (BL) was induced with toluene solution (26.5 mg L-1) for 2 weeks. Longevity of inductions of the biosensor was shown in over 135 days trial, when the biosensor was repetitively induced with toluene solution (26.5 mg L-1) 68 times. The intensities of BL gradually decreased due to release of the adsorbed cells and they were only temporarily restored by the addition of nutrients. The intensities of BL induced with contaminated ground water were lower than in the mineral medium (MSM) with the same content of toluene. Ideal shape of OFE, which maximizes the detected signal, was calculated to be a Frustrum cone. The developed sensor is plausible for multiple and fast online monitoring of the presence of a pollutant in remote localities but not its concentration.
Trvalý link: http://hdl.handle.net/11104/0323569