A Preliminary Characterization of a Novel Recombinant Clostridial Collagenase Blend

0547273 - ÚOCHB 2022 RIV CZ eng J - Článek v odborném periodiku
Leontovyc, I. - Koblas, T. - Berková, Z. - Bittenglová, K. - Leontovyč, Adrian - Benešík, M. - Saudek, F.
A Preliminary Characterization of a Novel Recombinant Clostridial Collagenase Blend.
Folia Biologica. Roč. 67, č. 2 (2021), s. 82-89. ISSN 0015-5500. E-ISSN 0015-5500
Institucionální podpora: RVO:61388963
Klíčová slova: collagenase * recombinant * Escherichia coli
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 1.167, rok: 2021
Způsob publikování: Open access
https://fb.cuni.cz/file/5950/fb2021a0010.pdf

Background and purpose: Despite advances in endovascular therapies, some patients experience vertebral artery stenosis or subclavian artery occlusion and may not benefit from less-invasive angioplasty/stenting. This study described 4 cases in which carotid-vertebral transposition (CVT) or carotid-subclavian transposition (CST) was adapted when endovascular treatment was unfeasible or unsuccessful. Presentation: Case 1: A 65-year-old woman presented with severe stenosis of the right vertebral artery ostium, dysplastic left vertebral artery, and aneurysmal dilatation of proximal right subclavian artery and brachiocephalic trunk. Case 2: A 23-year-woman had severe stenosis at the first portion of left vertebral artery caused by Takayasu's arteritis. Because endovascular intervention was unfeasible, CVTs were performed in cases 1 and 2. Case 3: A 73-year-old man presented with total occlusion of the proximal right subclavian artery and severe stenosis of the right internal carotid artery. Case 4: A 58-year-old man experienced complete occlusion of the left subclavian artery and severe stenosis of the left common carotid artery. Duplex ultrasonography showed reverse flow in the vertebral Biocompatibility of the blend was validated by a standardized viability assay on the isolated islets. Two batches of rColGH were produced and compared to a commercially available collagenase. Based on our results, we conclude that rColGH is a functional and non-toxic novel recombinant collagenase worth further characterization and blend optimization in order to make it a competitive commercial product.
Trvalý link: http://hdl.handle.net/11104/0323548