Fluorescein isothiocyanate stability in different solvents

0547236 - ÚOCHB 2022 RIV AT eng J - Článek v odborném periodiku
Jaklová Dytrtová, Jana - Moslova, K. - Jakl, M. - Sirén, H. - Riekkola, M. L.
Fluorescein isothiocyanate stability in different solvents.
Monatshefte fur Chemie. Roč. 152, č. 11 (2021), s. 1299-1306. ISSN 0026-9247. E-ISSN 1434-4475
Grant CEP: GA MŠMT(CZ) EF16_027/0008477
Institucionální podpora: RVO:61388963
Klíčová slova: FITC labelling * isomerism * lactone form * quinoid form * ToF–MS
Obor OECD: Analytical chemistry
Impakt faktor: 1.613, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1007/s00706-021-02852-1

Well-established label in biomolecules analysis, fluorescein isothiocyanate is commercially available in its two different isomers with equal fluorescence sensitivity, differing in meta- vs. para-isothiocyanate position. It was found in this study that the stability of the isomers depends on the polarity of different solvents and the pyridine (stabiliser) presence. The lowest stability has fluorescein isothiocyanate in water solvent. In acidic solution, their quinoid (acid) form switches to the lactone form. The p-quinoid form has higher tendency to create dimers and trimers and is less soluble in acetone than the lactone form.
Trvalý link: http://hdl.handle.net/11104/0323521