Biphenyl 2,3-Dioxygenase in Pseudomonas alcaliphila JAB1 Is Both Induced by Phenolics and Monoterpenes and Involved in Their Transformation

0547230 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
Zubrová, A. - Michalíková, Klára - Semerád, Jaroslav - Strejček, M. - Cajthaml, Tomáš - Šuman, J. - Uhlík, O.
Biphenyl 2,3-Dioxygenase in Pseudomonas alcaliphila JAB1 Is Both Induced by Phenolics and Monoterpenes and Involved in Their Transformation.
Frontiers in Microbiology. Roč. 12, APR 30 2021 (2021), č. článku 657311. ISSN 1664-302X. E-ISSN 1664-302X
Grant CEP: GA ČR(CZ) GA17-00227S
Institucionální podpora: RVO:61388971
Klíčová slova: secondary plant metabolites * aromatic ring-hydroxylating dioxygenases * biphenyl dioxygenase * phenolics * monoterpenes
Obor OECD: Environmental biotechnology
Impakt faktor: 6.064, rok: 2021
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fmicb.2021.657311/full

The involvement of bacterial aromatic ring-hydroxylating dioxygenases (ARHDs) in the degradation of aromatic pollutants, such as polychlorinated biphenyls (PCBs), has been well studied. However, there is considerable speculation as to the origin of this ability. One hypothesis is centered on a connection between the ability to degrade aromatic pollutants and the necessity of soil bacteria to cope with and/or utilize secondary plant metabolites (SPMs). To investigate this connection, we researched the involvement of biphenyl 2,3-dioxygenase (BPDO), an ARHD essential for the degradation of PCBs, in the metabolism of SPMs in the soil bacterium Pseudomonas alcaliphila JAB1, a versatile degrader of PCBs. We demonstrated the ability of the strain JAB1 to transform a variety of SPMs, namely the flavonoids apigenin, flavone, flavanone, naringenin, fisetin, quercetin, morin, and catechin, caffeic acid, trans-cinnamic acid, and the monoterpenes (S)-limonene and (R)-carvone. Of those, the transformation of flavone, flavanone, and (S)-limonene was conditioned by the activity of JAB1-borne BPDO and thus was researched in more detail, and we found evidence for the limonene monooxygenase activity of the BPDO. Furthermore, the bphA gene in the strain JAB1 was demonstrated to be induced by a wide range of SPMs, with monoterpenes being the strongest inducers of the SPMs tested. Thus, our findings contribute to the growing body of evidence that ARHDs not only play a role in the catabolism of aromatic pollutants, but also of natural plant-derived aromatics, and this study supports the hypothesis that ARHDs participate in ecological processes mediated by SPMs.
Trvalý link: http://hdl.handle.net/11104/0323517