Thiolated poly(2-hydroxyethyl methacrylate) hydrogels as a degradable biocompatible scaffold for tissue engineering

0547091 - ÚMCH 2022 RIV CH eng J - Článek v odborném periodiku
Macková, Hana - Hlídková, Helena - Kaberova, Zhansaya - Proks, Vladimír - Kučka, Jan - Patsula, Vitalii - Vetrík, Miroslav - Janoušková, Olga - Podhorská, Bohumila - Pop-Georgievski, Ognen - Kubinová, Šárka - Horák, Daniel
Thiolated poly(2-hydroxyethyl methacrylate) hydrogels as a degradable biocompatible scaffold for tissue engineering.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 131, December (2021), č. článku 112500. ISSN 0928-4931. E-ISSN 1873-0191
Grant CEP: GA ČR(CZ) GA20-07015S; GA ČR(CZ) GA20-07313S
Institucionální podpora: RVO:61389013 ; RVO:68378041
Klíčová slova: poly(2-hydroxyethyl methacrylate) * 2-methacryloyloxyethyl phosphorylcholine * 2-(acetylthio)ethyl methacrylate
Obor OECD: Polymer science; Biophysics (UEM-P)
Impakt faktor: 8.457, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0928493121006408

Research of degradable hydrogel polymeric materials exhibiting high water content and mechanical properties resembling tissues is crucial not only in drug delivery systems but also in tissue engineering, medical devices, and biomedical-healthcare sensors. Therefore, we newly offer development of hydrogels based on poly(2-hydroxyethyl methacrylate-co-2-(acetylthio) ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] and optimization of their mechanical and in vitro and in vivo degradability. P(HEMA-ATEMA-MPC) hydrogels differed in chemical composition, degree of crosslinking, and starting molar mass of polymers (15, 19, and 30 kDa). Polymer precursors were synthesized by a reversible addition fragmentation chain transfer (RAFT) polymerization using 2-(acetylthio)ethyl methacrylate containing protected thiol groups, which enabled crosslinking and gel formation. Elastic modulus of hydrogels increased with the degree of crosslinking (Slaughter et al., 2009) [1]. In vitro and in vivo controlled degradation was confirmed using glutathione and subcutaneous implantation of hydrogels in rats, respectively. We proved that the hydrogels with higher degree of crosslinking retarded the degradation. Also, albumin, γ-globulin, and fibrinogen adsorption on P(HEMA-ATEMA-MPC) hydrogel surface was tested, to simulate adsorption in living organism. Rat mesenchymal stromal cell adhesion on hydrogels was improved by the presence of RGDS peptide and laminin on the hydrogels. We found that rat mesenchymal stromal cells proliferated better on laminin-coated hydrogels than on RGDS-modified ones.
Trvalý link: http://hdl.handle.net/11104/0323584