Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

0547077 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
Rusanov, A. L. - Kozhin, P. M. - Tikhonova, O.V. - Zgoda, V. G. - Loginov, Dmitry Sergej - Chlastáková, A. - Selinger, Martin - Štěrba, J. - Grubhoffer, Libor - Luzgina, N. G.
Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages.
International Journal of Molecular Sciences. Roč. 22, č. 12 (2021), č. článku 6323. E-ISSN 1422-0067
Grant CEP: GA MŠMT(CZ) LTARF18021
Institucionální podpora: RVO:61388971 ; RVO:60077344
Klíčová slova: regulates phagocytosis * activation * pmj2-r * peritoneal macrophages * phagocytosis * proteome * lc-ms * ms
Obor OECD: Biochemistry and molecular biology; Microbiology (BC-A)
Impakt faktor: 6.208, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/22/12/6323

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.
Trvalý link: http://hdl.handle.net/11104/0323419