Sub-minute analysis of lactate from a single blood drop using capillary electrophoresis with contactless conductivity detection in monitoring of athlete performance

0546268 - ÚIACH 2022 RIV CH eng J - Článek v odborném periodiku
Kubáň, Petr - Dosedělová, Věra - Martma, K. - Rannama, I. - Reinpold, K. - Shimmo, R.
Sub-minute analysis of lactate from a single blood drop using capillary electrophoresis with contactless conductivity detection in monitoring of athlete performance.
Molecules. Roč. 26, č. 19 (2021), č. článku 5817. E-ISSN 1420-3049
Grant ostatní: AV ČR(CZ) EST-18-03
Program: Bilaterální spolupráce
Institucionální podpora: RVO:68081715
Klíčová slova: blood plasma separation * capillary electrophoresis * training assessment
Obor OECD: Analytical chemistry
Impakt faktor: 4.927, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/1420-3049/26/19/5817

A simple and fast method for the analysis of lactate from a single drop of blood was developed. The finger-prick whole blood sample (10 µL) was diluted (1:20) with a 7% (w/v) solution of tris(hydroxymethyl)methylamino] propanesulfonic acid and applied to a blood plasma separation device. The device accommodates a membrane sandwich composed of an asymmetric polysulfone membrane and a supporting textile membrane that allows the collection of blood plasma into a narrow glass capillary in less than 20 s. Separated and simultaneously diluted blood plasma was directly injected into a capillary electrophoresis instrument with a contactless conductivity detector (CE-C4 D) and analyzed in less than one minute. A separation electrolyte consisted of 10 mmol/L L-histidine, 15 mmol/L DL-glutamic acid, and 30 µmol/L cetyltrimethylammonium bromide. The whole procedure starting from the finger-prick sampling until the CE-C4 D analysis was finished, took less than 5 min and was suitable for monitoring lactate increase in blood plasma during incremental cycling exercise. The observed lactate increase during the experiments measured by the developed CE-C4 D method correlated well with the results from a hand-held lactate analyzer (R = 0.9882). The advantage of the developed CE method is the speed, significant savings per analysis, and the possibility to analyze other compounds from blood plasma
Trvalý link: http://hdl.handle.net/11104/0322809