Targeted mass spectrometry for monitoring of neural differentiation

0546153 - ÚŽFG 2022 RIV GB eng J - Článek v odborném periodiku
Suchá, Rita - Kubíčková, Martina - Červenka, Jakub - Hruška-Plocháň, M. - Bohačiaková, D. - Vodičková Kepková, Kateřina - Nováková, Tereza - Budková, Kateřina - Šušor, Andrej - Maršala, M. - Motlík, Jan - Kovářová, Hana - Vodička, Petr
Targeted mass spectrometry for monitoring of neural differentiation.
Biology Open. Roč. 10, č. 8 (2021), č. článku bio058727. ISSN 2046-6390. E-ISSN 2046-6390
Grant CEP: GA MŠMT(CZ) LO1609; GA MŠMT(CZ) LTC18079; GA MŠMT(CZ) EF16_019/0000785
Institucionální podpora: RVO:67985904
Klíčová slova: neural stem cell * neural differentiation * selected reaction monitoring
Obor OECD: Biochemical research methods
Impakt faktor: 2.643, rok: 2021
Způsob publikování: Open access
https://journals.biologists.com/bio/article/10/8/bio058727/271174/Targeted-mass-spectrometry-for-monitoring-of

Human multipotent neural stem cells could effectively be used for the treatment of a variety of neurological disorders. However, a defining signature of neural stem cell lines that would be expandable, non-tumorigenic, and differentiate into desirable neuronal/glial phenotype after in vivo grafting is not yet defined. Employing a mass spectrometry approach, based on selected reaction monitoring, we tested a panel of well-described culture conditions, and measured levels of protein markers routinely used to probe neural differentiation, i.e. POU5F1 (OCT4), SOX2, NES, DCX, TUBB3, MAP2, S100B, GFAP, GALC, and OLIG1. Our multiplexed assay enabled us to simultaneously identify the presence of pluripotent, multipotent, and lineage committed neural cells, thus representing a powerful tool to optimize novel and highly specific propagation and differentiation protocols. The multiplexing capacity of this method permits the addition of other newly identified cell type-specific markers to further increase the specificity and quantitative accuracy in detecting targeted cell populations. Such an expandable assay may gain the advantage over traditional antibody-based assays, and represents a method of choice for quality control of neural stem cell lines intended for clinical use.
Trvalý link: http://hdl.handle.net/11104/0322705