Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria

0546092 - FZÚ 2022 RIV GB eng J - Článek v odborném periodiku
Obořilová, R. - Šimečková, H. - Pastucha, M. - Klimovič, Š. - Víšová, Ivana - Přibyl, J. - Vaisocherová-Lísalová, Hana - Pantůček, R. - Skládal, P. - Mašlaňová, I. - Farka, Z.
Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria.
Nanoscale. Roč. 13, č. 31 (2021), s. 13538-13549. ISSN 2040-3364. E-ISSN 2040-3372
Institucionální podpora: RVO:68378271
Klíčová slova: atomic force microscopy * AFM * surface plasmon resonance * SPR * Staphylococcus aureus * lysis * lysostaphin * bacteriophage * phage therapy
Obor OECD: Biophysics
Impakt faktor: 8.307, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1039/d1nr02921e

The growing incidence of multidrug-resistant bacterial strains presents a major challenge in modern medicine. Antibiotic resistance is often exhibited by Staphylococcus aureus, which causes severe infections in human and animal hosts and leads to significant economic losses. Antimicrobial agents with enzymatic activity (enzybiotics) and phage therapy represent promising and effective alternatives to classic antibiotics. However, new tools are needed to study phage–bacteria interactions and bacterial lysis with high resolution and in real-time. Here, we introduce a method for studying the lysis of S. aureus at the single-cell level in real-time using atomic force microscopy (AFM) in liquid. We demonstrate the ability of the method to monitor the effect of the enzyme lysostaphin on S. aureus and the lytic action of the Podoviridae phage P68.
Trvalý link: http://hdl.handle.net/11104/0322681