Počet záznamů: 1
Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo
- 1.0546076 - ÚŽFG 2022 RIV GB eng J - Článek v odborném periodiku
Jansová, Denisa - Aleshkina, Daria - Jindrová, Anna - Iyyappan, Rajan - An, Q. - Fan, G. - Šušor, Andrej
Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo.
Journal of Molecular Biology. Roč. 433, č. 19 (2021), č. článku 167166. ISSN 0022-2836. E-ISSN 1089-8638
Grant CEP: GA ČR GA18-19395S
Institucionální podpora: RVO:67985904
Klíčová slova: mRNA * translation * oocyte
Obor OECD: Developmental biology
Impakt faktor: 6.151, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S002228362100396X?via%3Dihub
During oocyte growth the cell accumulates RNAs to contribute to oocyte and embryo development which progresses with ceased transcription. To investigate the subcellular distribution of specific RNAs and their translation we developed a technique revealing several instances of localized translation with distinctive regulatory implications. We analyzed the localization and expression of candidate non-coding and mRNAs in the mouse oocyte and embryo. Furthermore, we established simultaneous visualization of mRNA and in situ translation events validated with polysomal occupancy. We discovered that translationally dormant and abundant mRNAs CyclinB1 and Mos are localized in the cytoplasm of the fully grown GV oocyte forming cloud-like structures with consequent abundant translation at the center of the MII oocyte. Coupling detection of the localization of specific single mRNA molecules with their translation at the subcellular context is a valuable tool to quantitatively study temporal and spatial translation of specific target mRNAs to understand molecular processes in the developing cell.
Trvalý link: http://hdl.handle.net/11104/0322669
Počet záznamů: 1