Advanced glycosidases as ingenious biosynthetic instruments

0546051 - MBÚ 2022 RIV US eng J - Článek v odborném periodiku
Mészáros, Zuzana - Nekvasilová, Pavlína - Bojarová, Pavla - Křen, Vladimír - Slámová, Kristýna
Advanced glycosidases as ingenious biosynthetic instruments.
Biotechnology Advances. Roč. 49, JUL-AUG (2021), č. článku 107733. ISSN 0734-9750. E-ISSN 1873-1899
Grant CEP: GA ČR(CZ) GA20-00477S; GA ČR(CZ) GA20-00215S; GA MŠMT(CZ) LTC19035; GA MŠMT(CZ) LTC20069; GA MŠMT(CZ) LTC20072
Institucionální podpora: RVO:61388971
Klíčová slova: beta-N-Acetylhexosaminidase * Chitinase * Enzyme engineering * alpha-L-Fucosidase * beta-Galactosidase * Glycosidase * Glycosynthase * Oligosaccharide * Thioglycoligase * Transglycosylation
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 17.681, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0734975021000392?via%3Dihub

Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found few synthetic applications in industry, being primarily used for cleaving unwanted carbohydrates. With the establishment of glycosynthase and transglycosidase technology by genetic engineering, the view of glycosidases as industrial biotechnology tools has started to change. Their easy production, affordability, robustness, and substrate versatility, added to the possibility of controlling undesired side hydrolysis by enzyme engineering, have made glycosidases competitive synthetic tools. Current promising applications of engineered glycosidases include the production of well-defined chitooligomers, precious galactooligosaccharides or specialty chemicals such as glycosylated flavonoids. Other synthetic pathways leading to human milk oligosaccharides or remodeled antibodies are on the horizon. This work provides an overview of the synthetic achievements to date for glycosidases, emphasizing the latest trends and outlining possible developments in the field.
Trvalý link: http://hdl.handle.net/11104/0322621