The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition

0545934 - ÚVGZ 2022 RIV US eng J - Článek v odborném periodiku
Sharma, N. - Sahu, Pranav P. - Prasad, A. - Muthamilarasan, M. - Waseem, M. - Khan, Y. - Thakur, J. K. - Chakraborty, S. - Prasad, M.
The Sw5a gene confers resistance to ToLCNDV and triggers an HR response after direct AC4 effector recognition.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 118, č. 33 (2021), č. článku e2101833118. ISSN 0027-8424. E-ISSN 1091-6490
Výzkumná infrastruktura: CzeCOS III - 90123
Institucionální podpora: RVO:86652079
Klíčová slova: delhi-virus-infection * cell-death * micrornas * tomato * mir159 * mirna * myb * expression * prediction * target * hypersensitive response * microRNAs * Myb transcription factor * Sw5  * gene * Tomato leaf curl virus
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 12.779, rok: 2021
Způsob publikování: Omezený přístup
https://www.pnas.org/content/118/33/e2101833118

Several attempts have been made to identify antiviral genes against Tomato leaf curl New Delhi virus (ToLCNDV) and related viruses. This has led to the recognition of Ty genes (Ty1-Ty6), which have been successful in developing virus-resistant crops to some extent. Owing to the regular appearance of resistance-breaking strains of these viruses, it is important to identify genes related to resistance. In the present study, we identified a ToLCNDV resistance (R) gene, SlSw5a, in a ToLCNDV-resistant tomato cultivar, H-88-78-1, which lacks the known Ty genes. The expression of SlSw5a is controlled by the transcription factor SlMyb33, which in turn is regulated by microRNA159 (sly-miR159). Virus-induced gene silencing of either SlSw5a or SlMyb33 severely increases the disease symptoms and viral titer in leaves of resistant cultivar. Moreover, in SlMyb33-silenced plants, the relative messenger RNA level of SlSw5a was reduced, suggesting SlSw5a is downstream of the sly-miR159-SlMyb33 module. We also demonstrate that SlSw5a interacts physically with ToLCNDV-AC4 (viral suppressor of RNA silencing) to trigger a hypersensitive response (HR) and generate reactive oxygen species at infection sites to limit the spread of the virus. The ´RTSK´ motif in the AC4 C terminus is important for the interaction, and its mutation completely abolishes the interaction with Sw5a and HR elicitation. Overall, our research reports an R gene against ToLCNDV and establishes a connection between the upstream miR159-Myb33 module and its downstream target Sw5a to activate HR in the tomato, resulting in geminivirus resistance.
Trvalý link: http://hdl.handle.net/11104/0322553