Bodipy conjugate of epibrassinolide as a novel biologically active probe for in vivo imaging

0545887 - ÚEB 2022 RIV CH eng J - Článek v odborném periodiku
Starodubtseva, Anastasiia - Kalachova, Tetiana - Iakovenko, Oksana - Stoudková, Věra - Zhabinskii, V.N. - Khripach, V. - Ruelland, E. - Martinec, Jan - Burketová, Lenka - Kravets, V.
Bodipy conjugate of epibrassinolide as a novel biologically active probe for in vivo imaging.
International Journal of Molecular Sciences. Roč. 22, č. 7 (2021), č. článku 3599. E-ISSN 1422-0067
Grant CEP: GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) LM2018129
Institucionální podpora: RVO:61389030
Klíčová slova: Brassinosteroids * Fluorescent conjugates * Live imaging * Plant bioassay
Obor OECD: Biochemical research methods
Impakt faktor: 6.208, rok: 2021
Způsob publikování: Open access
http://doi.org/10.3390/ijms22073599

Brassinosteroids (BRs) are plant hormones of steroid nature, regulating various developmental and adaptive processes. The perception, transport, and signaling of BRs are actively studied nowadays via a wide range of biochemical and genetic tools. However, most of the knowledge about BRs intracellular localization and turnover relies on the visualization of the receptors or cellular compartments using dyes or fluorescent protein fusions. We have previously synthesized a conjugate of epibrassinolide with green fluorescent dye BODIPY (eBL-BODIPY). Here we present a detailed assessment of the compound bioactivity and its suitability as probe for in vivo visualization of BRs. We show that eBL-BODIPY rapidly penetrates epidermal cells of Arabidopsis thaliana roots and after long exposure causes physiological and transcriptomic responses similar to the natural hormone.
Trvalý link: http://hdl.handle.net/11104/0322513