The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence

0545863 - ÚEB 2022 RIV GB eng J - Článek v odborném periodiku
Karafiátová, Miroslava - Bednářová, Martina - Said, Mahmoud - Čížková, Jana - Holušová, Kateřina - Blavet, Nicolas - Bartoš, Jan
The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence.
Journal of Experimental Botany. Roč. 72, č. 5 (2021), s. 1606-1616. ISSN 0022-0957. E-ISSN 1460-2431
Grant CEP: GA ČR(CZ) GJ18-12338Y; GA MŠMT(CZ) EF16_019/0000827; GA MŠMT(CZ) LM2015047
Institucionální podpora: RVO:61389030
Klíčová slova: B chromosomes * cytogenetics * flow cytometry * pollen nuclei * repeat analysis * Sorghum purpureosericeum
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 7.378, rok: 2021
Způsob publikování: Open access
http://doi.org/10.1093/jxb/eraa548

More than a century has passed since the B chromosomes were first discovered. Today we know much of their variability, morphology, and transmission to plant progeny. With the advent of modern technologies, B chromosome research has accelerated, and some of their persistent mysteries have since been uncovered. Building on this momentum, here we extend current knowledge of B chromosomes in Sorghum purpureosericeum to the sequence level. To do this, we estimated the B chromosome size at 421 Mb, sequenced DNA from flow-sorted haploid pollen nuclei of both B-positive (B+) and B-negative (B0) plants, and performed a repeat analysis on the Illumina raw sequence data. This analysis revealed nine putative B-specific clusters, which were then used to develop B chromosome-specific markers. Additionally, cluster SpuCL4 was identified and verified to be a centromeric repeat. We also uncovered two repetitive clusters (SpuCL168 and SpuCL115), which hybridized exclusively on the B chromosome under fluorescence in situ hybridization and can be considered as robust cytogenetic markers. Given that B chromosomes in Sorghum are rather unstable across all tissues, our findings could facilitate expedient identification of B+ plants and enable a wide range of studies to track this chromosome type in situ.
Trvalý link: http://hdl.handle.net/11104/0322497