Optical sensors of heterotrimeric G protein signaling

0545820 - MBÚ 2022 RIV GB eng J - Článek v odborném periodiku
Bondar, Alexey - Lazar, Josef
Optical sensors of heterotrimeric G protein signaling.
FEBS Journal. Roč. 288, č. 8 (2021), s. 2570-2584. ISSN 1742-464X. E-ISSN 1742-4658
Grant CEP: GA ČR(CZ) GJ17-14413Y; GA ČR(CZ) GJ20-09628Y; GA MŠMT(CZ) LTC20074
Institucionální podpora: RVO:61388971
Klíčová slova: bret * fret * G protein * G protein-coupled receptor * imaging * optogenetics * polarization microscopy * sensor * single-molecule imaging * tirf
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.622, rok: 2021
Způsob publikování: Open access
https://febs.onlinelibrary.wiley.com/doi/epdf/10.1111/febs.15655

Heterotrimeric G proteins are central mediators of cellular signal transduction. They receive, process, and transduce signals from G protein-coupled receptors to downstream effectors. Since their discovery, a number of optical sensors of G protein localisation and function have been developed and applied in living systems. In this minireview, we provide an overview of existing G protein-based sensors and the experimental approaches they utilise, with emphasis on live-cell imaging techniques. We outline recent advances, as well as identify current challenges and likely future directions in the field of G protein sensor development.
Trvalý link: http://hdl.handle.net/11104/0322466