Počet záznamů: 1  

Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga Parachlorella kessleri

  1. 1.
    0545771 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
    Zachleder, Vilém - Kselíková, Veronika - Ivanov, Ivan - Bialevich, Vitali - Vítová, Milada - Ota, S. - Takeshita, T. - Kawano, S. - Bišová, Kateřina
    Supra-Optimal Temperature: An Efficient Approach for Overaccumulation of Starch in the Green Alga Parachlorella kessleri.
    Cells. Roč. 10, č. 7 (2021), č. článku 1806. E-ISSN 2073-4409
    Grant CEP: GA ČR(CZ) GA17-06264S
    Institucionální podpora: RVO:61388971
    Klíčová slova: microalgae * Parachlorella kessleri * starch * supra-optimal temperature * cell cycle * energy reserves * growth processes * reproduction events * deuterium * deuterated starch * deuterated lipid
    Obor OECD: Cell biology
    Impakt faktor: 7.666, rok: 2021
    Způsob publikování: Open access
    https://www.mdpi.com/2073-4409/10/7/1806

    Green algae are fast-growing microorganisms that are considered promising for the production of starch and neutral lipids, and the chlorococcal green alga Parachlorella kessleri is a favorable model, as it can produce both starch and neutral lipids. P. kessleri commonly divides into more than two daughter cells by a specific mechanism-multiple fission. Here, we used synchronized cultures of the alga to study the effects of supra-optimal temperature. Synchronized cultures were grown at optimal (30 degrees C) and supra-optimal (40 degrees C) temperatures and incident light intensities of 110 and 500 mu mol photons m(-2) s(-1). The time course of cell reproduction (DNA replication, cellular division), growth (total RNA, protein, cell dry matter, cell size), and synthesis of energy reserves (net starch, neutral lipid) was studied. At 40 degrees C, cell reproduction was arrested, but growth and accumulation of energy reserves continued, this led to the production of giant cells enriched in protein, starch, and neutral lipids. Furthermore, we examined whether the increased temperature could alleviate the effects of deuterated water on Parachlorella kessleri growth and division, results show that supra-optimal temperature can be used in algal biotechnology for the production of protein, (deuterated) starch, and neutral lipids.
    Trvalý link: http://hdl.handle.net/11104/0322433

     
     
Počet záznamů: 1  

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